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KMID : 0351619740150020195
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Kyungpook Medical Journal
1974 Volume.15 No. 2 p.195 ~ p.202
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Studies on strains of Salmonella typhi requiring tryptophane for growth in synthetic medium
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Abstract
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When isolated and standard strains of Salmonella typhi were inoculated into a synthetic medium composed of salts and casamino acid, about an half of the test strains were unable to grow.
Among amino acid, vitamine, glucose and nucleic acid substance, exogenous tryptophane was a unique requirement for growth of these auxotrophes although some quantitative difference in requirement was noted by the strains.
Various sizes of minute colonies were observed microscopically with irregular cell morphology of coccobacillary to filamentous forms when tryptophane requiring strains were cultured on solid synthetic medium with rare segregation of tryptophane non-requiring mutants.
These tryptophane requiring strains were somewhat less sensitive to tetracycline, chlorampenicol, ampicillin and nalidixic acid as compared to tryptophane-non requiring strains, this characteristic was confirmed when tryptophane non-requiring mutants which were segregated from requiring strains were compared with parent strains.
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